Composite
pPQS-GFP

Part:BBa_K1157017:Design

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)


pPQS-GFPmut3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 299
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1014


Design Notes

There are no restriction sites in the inner part of the DNA so there are no specific design to the sequence.


Source

pPQS is originally made through PCR of whole genome Pseudomonas aeruginosa ATCC 27853. The reporter is constructed by igem biobricks from iGEM official.

References